以往确定小麦醇溶蛋白和高分子及低分子麦谷蛋白的亚基组分,需要3个独立的单向电泳分离过程.本文所述及的方法只需要两个电泳步骤.用2-氯乙醇提取蛋白质之后,先在酸性条件下进行聚丙烯酰胺凝胶电泳(A-PAGE)分离,测定麦醇溶蛋白组分.然后,将位于A-PAGE凝胶板点样槽紧下面的几个毫米内的非还原聚合蛋白经过还原并用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)分离,这些蛋白质便可产生反映麦谷蛋白高分子和低分子亚基特性的多肽模式. In the past, the determination of the subunit components of wheat gliadin and polymer and low-molecular-weight glutenin required three separate one-dimensional electrophoresis separations. The method described herein requires only two electrophoresis steps. Extraction with 2-chloroethanol After protein separation, polyacrylamide gel electrophoresis (A-PAGE) was first performed under acidic conditions to determine the gliadin fraction, and the gliadin fraction was then placed within a few millimeters immediately below the point on the A-PAGE gel plate Of nonreducing polymeric proteins are reduced and separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). These proteins produce polypeptide patterns reflecting the properties of glutenin macromolecules and low molecular subunits.