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目的研究己酮可可碱(PTX)对大鼠全脑缺血-再灌注(I-R)损伤的保护作用。方法 36只雄性SD大鼠随机均分为3组:A组假手术对照,B组和C组分别于再灌注时腹腔注射生理盐水10 ml/kg和PTX 100 mg/kg,24 h后检测脑皮层核因子-κB(NF-κB)活性、半胱天冬氨酸蛋白酶3(Caspase-3)表达以及细胞凋亡情况。结果 B组和C组中脑皮层NF-κB的活性比A组增强[(7.95±0.69)、(3.89±0.35)vs.(1.12±0.17)]、Caspase-3蛋白表达增加[(1.387±0.129)、(0.786±0.062)vs.(0.416±0.052)]、TUNEL阳性细胞数增加[(34.87±3.68)(、17.85±2.71)vs.(6.13±1.54)](P<0.05)。C组上述各项观察指标均较B组显著降低(P<0.05)。结论 PTX能够有效减轻全脑I-R后大鼠脑的细胞凋亡和炎症反应,进而减轻再灌注损伤。
Objective To investigate the protective effect of pentoxifylline (PTX) on global cerebral ischemia-reperfusion (I-R) injury in rats. Methods Thirty-six male Sprague-Dawley rats were randomly divided into three groups: sham control group A, B group and C group were injected intraperitoneally with saline (10 ml / kg) and PTX 100 mg / kg Cortical nuclear factor-κB (NF-κB) activity, Caspase-3 expression and apoptosis. Results The activity of NF-κB in middle and cerebral cortex of group B and group C was significantly higher than that of group A [(7.95 ± 0.69) vs. (3.89 ± 0.35) vs. (1.12 ± 0.17) vs. (1.387 ± 0.129 ), (0.786 ± 0.062) vs. (0.416 ± 0.052), respectively. The number of TUNEL-positive cells increased (34.87 ± 3.68 vs. 17.85 ± 2.71 vs 6.13 ± 1.54, P <0.05). The above indexes in group C were significantly lower than those in group B (P <0.05). Conclusion PTX can effectively reduce the brain apoptosis and inflammatory response after I-R in rats, and then reduce the reperfusion injury.