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Swelling_inducedCl_currentswereinvestigatedinfreshlypreparednon_pigmentedepithelial(NPE)andpigmentedepithe lial(PE)cellsoftherabbitciliarybodyusingthewhole_cellpatchclamptechnique .ExposureofbothNPEandPEcellstohypotonic stressinducedCl_currentsthatexhibitedoutwardrectificationandwereinsensitivetoCa2 +.Wefoundthatswelling_inducedCl_cur rentsinPEcellareobservedshortlyafterisolation .Theswelling_inducedCl_currentshowlittleornoinactivationatpositivemembrane voltagesandwassensitiveto 10 0microMNPPBand 10 0microM .InjectionofcRNAencodedrabbitpIClnintoXenopusoocytespro ducedanoutwardlyrectifyingCl_currentdisplayingfeaturesconsistentwiththeswelling_inducedCl_currentinepithelium .pIClnisu biquitousintheciliaryepithelium .Itparticipatesintheequilibrationofshorttermtonicityalterations,aphenomenonunderlyingmech anismswithlargerandsloweramplitudesforaqueoussecretionbythesecells .
Swelling_inducedCl_currentswereinvestigatedinfreshlypreparednon_pigmentedepithelial (NPE) andpigmentedepithe lial (PE) cellsoftherabbitciliarybodyusingthewhole_cellpatchclamptechnique .ExposureofbothNPEandPEcellstohypotonic stressinducedCl_currentsthatexhibitedoutwardrectificationandwereinsensitivetoCa2 + .Wefoundthatswelling_inducedCl_cur rentsinPEcellareobservedshortlyafterisolation .Theswelling_inducedCl_currentshowlittleornoinactivationatpositivemembrane voltagesandwassensitiveto 10 0microMNPPBand 10 0microM .InjectionofcRNAencodedrabbitpIClnintoXenopusoocytespro ducedanoutwardlyrectifyingCl_currentdisplayingfeaturesconsistentwiththeswelling_inducedCl_currentinepithelium .pIClnisu biquitousintheciliaryepithelium .Itparticipatesintheequilibrationofshorttermtonicityalterations, aphenomenonunderlyingmech anismswithlargerandsloweramplitudesforaqueoussecretionbythesecells.