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从担子菌灰树花的菌丝体中提取总ＲＮＡ，并纯化出ｍＲＮＡ，经ＲＴ－ＰＣＲ扩增得到一长约２．２ｋｂ的片段，经测序，其全长２１９９ ｂｐ，包括起始密码和终止密码；随后将此片段插入植物表达载体ｐＢＩ１２１的３５Ｓ启动子和ＮＯＳ终止子之间，构建了一植物表达载体（ｐＢＴ），又将此片段加上ｕｂｉ－Ｌ启动子和ＮＯＳ终止子构建了另一植物表达载体（ｐＵＢＴ），然后通过基因枪法分别用植物表达载体ｐＢＴ和ｐＵＢＴ转化甘蔗，再生植株的点杂交和ＰＣＲ－Ｓｏｕｔｈｅｒｎ杂交证明海藻糖合酶基因已整合进甘蔗的基因组中。
The total RNA was extracted from the mycelium of M. grifola frondosa and its mRNA was purified. A 2.2 kb fragment was amplified by RT-PCR and sequenced. The full-length cDNA was 2199 bp in length, including the start codon and Followed by the insertion of the fragment between the 35S promoter and the NOS terminator of the plant expression vector pBI121, a plant expression vector (pBT) was constructed, and this fragment was added with the ubi-L promoter and the NOS terminator Another plant expression vector (pUBT) was constructed and then transformed into plant sugarcane by using the plant expression vectors pBT and pUBT respectively. The dot blot and PCR-Southern hybridization of the regenerated plants proved that the trehalose synthase gene was integrated into the genome of sugarcane.