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本实验的目的在于了解毛蚶被污染了甲型肝炎病毒后经通常煮沸食用的方法能否全部杀灭病毒。由于病毒主要积聚在贝类的消化系统,本实验人工接种10~5TCID_(50)的甲型肝炎病毒于活毛蚶的胃肠区,经过煮沸5、10、15、20、25、30和45min的处理后,测定毛蚶中甲型肝炎病毒的存活情况。毛蚶中甲型肝炎病毒的提取采用经改良的Johnson方法。病毒检测采用SL_7细胞,培养4至5周后用间接免疫荧光法检测感染细胞中的甲型肝炎病毒抗原。结果表明,煮沸45min后毛蚶中小部分病毒仍能存活,尽管此时接种标本的8管细胞中只有2管阳性,阳性细胞所占百分比仅为3.8％。
The purpose of this experiment is to understand whether the catfish is contaminated with the hepatitis A virus and whether the virus can be completely killed after being boiled. As the virus mainly accumulates in the digestive system of shellfish, the experiment artificially inoculated 10 ~ 5TCID_ (50) of Hepatitis A virus in the gastrointestinal area of live hamster, after boiled for 5,10,15,20,25,30 and 45min After treatment, the survival of Hepatitis A virus was determined. Extraction of Hepatitis A virus in the catfish using a modified Johnson method. Virus detection using SL_7 cells, cultured 4 to 5 weeks after infection by indirect immunofluorescence detection of hepatitis A virus antigen. The results showed that 45 min after boiling, the virus still could survive in the catfish, although only 2 of the 8 cells inoculated with the virus were positive and the percentage of positive cells was only 3.8%.