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目的:对丽水市鼠肝脾组织进行巴尔通体检测,为控制人巴尔通体流行提供参考依据。方法:收集丽水市鼠的肝脾标本,采用PCR法检测巴尔通体gltA基因片段(379 bp),确定阳性标本。结果:在丽水市捕获鼠467只,巴尔通体总感染率为15.20%,宿主动物有黑线姬鼠、社鼠、小家鼠。黑线姬鼠为优势鼠种(429/467),巴尔通体感染率为16.08%(69/429)。结论:丽水市鼠中存在巴尔通体自然感染,且宿主动物呈多样性,为进一步深入进行巴尔通体病疫源地的调查奠定了基础。
OBJECTIVE: To detect Bartonella in hepatopulmonary and spleen tissues of rats in Lishui City, and to provide a reference for controlling the prevalence of Bartonella in vivo. Methods: The liver and spleen samples from Lishui rats were collected. The gltA gene fragment (379 bp) was amplified by PCR and the positive samples were determined. Results: 467 mice were captured in Lishui City. The total infection rate of Bartonella was 15.20%. The host animals were Apodemus agrarius, Salsa and Mus musculus. Apodemus agrarius was dominant species (429/467), Bartonella infection rate was 16.08% (69/429). Conclusion: The natural infection of Bartonella in Lishui rats and the diversity of host animals lay the foundation for further investigation of the epidemic area of Balton’s disease.