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本文报导了用酸性磷酸酶经溶酶体膜漏出作为体外石英对细胞毒性的指标,研究P_(204)(聚乙烯吡啶氮氧化合物)和聚乙烯吡咯烷酮的抗石英细胞毒的作用。实验用豚鼠腹腔巨噬细胞进行。先给豚鼠腹腔注入含0.4%P_(204)的盐水溶液,然后采集腹腔巨噬细胞;或采集未经该品处理的豚鼠腹腔巨噬细胞,在培养时加入含0.04%P_(204)的Tyrode溶液。并以未经任何处理的正常巨噬细胞为对照。尔后,在这些巨噬细胞培养基中加入裸露的石英颗粒或外裹P_(204)的石英颗粒。结果发现,未经P_(204)预处理的
This article reports the use of acid phosphatase lysosomal membrane leakage as an indicator of cytotoxicity in vitro Quartz to study the P_ (204) (polyvinylpyridine oxynitride) and polyvinylpyrrolidone anti-quartz cytotoxicity. Experiments were performed with guinea pig peritoneal macrophages. First, the guinea pigs were injected intraperitoneally with 0.4% P (204) saline solution, and then collected peritoneal macrophages; or without the product of guinea pig peritoneal macrophages collected during the culture, adding 0.04% P_ (204) Tyrode Solution. Normal macrophages without any treatment were used as controls. Thereafter, bare quartz particles or outer P_ (204) quartz particles were added to these macrophage media. The results showed that without P_ (204) pretreatment