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在研究小剂量X射线照射对全淋巴细胞刺激效应的同时,探讨了小剂量照射(0,0.02,0.05,0.10,0.20,0.50Gy)对三种单克隆抗体分离出的CD_4~+、CD_8~+和B亚群细胞的刺激效应,观察了小剂量照射后的CD_4~+、CD_8~+细胞对B细胞功能的影响。结果表明,Panning法分离的各亚群细胞的纯度和存活率均在90％以上,全淋巴细胞在0.10Gy剂量内有刺激作用,尤以0.10Gy剂量点效应最大(为对照组的139.2％);各亚群细胞在0.20Gy照射内,均有刺激效应的发生,CD_4~+和B细胞在0.10Gy剂量点效应最强(为对照的179.3％和182.5％),而CD_8~+在0.05Gy点效应最强(为对照的169.6％);相同剂量,CD_4~+和B细胞的刺激效应大于CD_8~+。0.50Gy照射则表现出明显的抑制作用。正常CD_4~+细胞对B细胞具有辅助功能,CD_8~+细胞对B细胞具有抑制作用,小剂量照射可加强CD_4~+细胞对B细胞的辅助作用,促进CD_8~+细胞对B细胞的抑制功能,以0.10Gy照射后这种效应最强,0.20Gy照后仍可观察到这种效应。
In the study of low dose X-ray irradiation on the whole lymphocyte stimulating effect at the same time to explore the low dose irradiation (0,0.02,0.05,0.10,0.20,0.50Gy) on the three monoclonal antibodies isolated CD_4 ~ +, CD_8 ~ + And B subpopulations. The effect of CD_4 ~ + and CD_8 ~ + cells on the function of B cells after low dose irradiation was observed. The results showed that the purity and survival rate of all the subpopulations separated by Panning method were over 90%, and the whole lymphocytes stimulated at 0.10Gy dose, especially the 0.10Gy dose point (139.2% of the control group) ; All subpopulations of cells had a stimulating effect at 0.20Gy irradiation, CD_4 ~ + and B cells had the strongest effect at 0.10Gy dose (179.3% and 182.5% of the control), while CD_8 + at 0.05Gy The strongest point effect (169.6% of the control); the same dose, CD_4 ~ + and B cells stimulating effect is greater than CD_8 ~ +. 0.50Gy irradiation showed a significant inhibitory effect. Normal CD_4 ~ + cells have the auxiliary function to B cells, CD_8 ~ + cells have inhibitory effect on B cells, and low dose irradiation can enhance the auxiliary effect of CD_4 ~ + cells on B cells and promote the inhibitory function of CD_8 ~ + cells on B cells , This effect is the strongest after irradiation with 0.10Gy, this effect can still be observed after 0.20Gy irradiation.