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目的 探讨霍乱毒素 (CTX)对金黄地鼠视网膜节细胞 (RGCs)再生的促进作用。 方法 成年金黄地鼠近端切断视神经 (ON )并缝接一段自体坐骨神经 (AG) ,玻璃体内注射 CTX 及 /或插入小段坐骨神经分支 (SN)。动物随机分为 AG组和溶剂组 ;AG+ CTX组 ;AG+ SN组 ;AG+ CTX+ SN组 ;量效关系组。前 4组动物存活 2～ 6周。用粒蓝逆行标记再生的 RGCs,在荧光镜下观察视网膜平铺片再生 RGCs的数量变化。 结果 AG+ CTX 组各时间点视网膜再生 RGCs平均数比 AG组和溶剂组增加 ,具有统计学意义 (P<0 .0 5 ) ;AG+ SN组也得出相似结果。AG+ CTX+ SN组各时间点的视网膜再生 RGCs平均数分别比 AG组和 AG+ SN组明显增加 (P<0 .0 1)。 结论 提示霍乱毒素或 /与坐骨神经具有显著促进视神经切断后视网膜节细胞再生的作用。
Objective To investigate the promoting effect of cholera toxin (CTX) on the regeneration of retinal ganglion cells (RGCs) in golden hamster. Methods The adult golden hamster cut off the optic nerve (ON) proximally and sutured an autologous sciatic nerve (AG). Intravitreal CTX injection and / or insertion of a short segment of the sciatic nerve branch (SN) were performed. The animals were randomly divided into AG group and solvent group; AG + CTX group; AG + SN group; AG + CTX + SN group; The first 4 groups of animals survived 2 to 6 weeks. Regenerated RGCs were labeled retrogradely with granulocytes and the number of RGCs regenerated from retinal patches was observed under fluoroscopy. Results The average number of RGCs regenerated in AG + CTX group at each time point was significantly higher than that in AG group and solvent group (P <0.05). Similar results were obtained in AG + SN group. The average number of RGCs regenerated in AG + CTX + SN group at each time point was significantly higher than that in AG group and AG + SN group (P <0.01). Conclusions The results suggest that cholera toxin or / and sciatic nerve may significantly promote retinal ganglion cell regeneration after optic nerve transection.