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AIM:To study the effect of probiotics on interleukin-8secretion in intestinal epithelia when stimulated byproinflammatory cytokines.METHODS:Colonic adenocardnoma HT29 cells were culturedand divided into four groups:control,TNF-α (group T in short),bifidobactedurn (group B),lactobacillus (group L).B.Longumand L.bulgaricus were suspended in culture medium with aconcentration of 1×10~8 cfu/ml and added into 24 wellsrespectively.One hour later TNF-α(10 ng/ml) was addedinto each well of groups T,B,L.The supernatants werecollected and measured for IL-8 after 3 hours,nuclear factor-кB (NF-кB) p65 was also examined by Western blotting.RESULTS:There was less interleukin-8 secretion in HT29cells when preincubated with B.Longum or L.bulgaricuscompared with group T.Less p65 appeared in nudei in groupsB and L compared with group T,as detected by Western blot.CONCLUSION:Probiotics can suppress intedeuldn-8 secretionin intestinal epithelia when stimulated by proinflammatorycytokines,which is most likely mediated by NF-кB.
AIM: To study the effect of probiotics on interleukin-8 secretion in intestinal epithelia when stimulated by proinflammatory cytokines. METHODS: Colonic adenocardium HT29 cells were cultured and divided into four groups: control, TNF-α (group T in short), bifidobactedurn (group B) , Lactobacillus (group L). B. Longumand L. bulgaricus were suspended in culture medium with aconcentration of 1 × 10-8 cfu / ml and added into 24 wells lastly. One hour later TNF-α (10 ng / ml) was addedinto each well of groups T, B, L. The supernatants werecollected and measured for IL-8 after 3 hours, nuclear factor-кB (NF-кB) p65 was also examined by Western blotting .RESULTS: There was less interleukin-8 secretion in HT29cells when preincubated with B. Longum or L. bulgaricus compared with group T. Less p65 appeared in nudei in groups B and L compared with group T, as detected by Western blot. CONCLUSION: Probiotics can suppress intedeuldn-8 secretion in intestinal epithelia when stimulated by proinflammatorycytokines, which is mos t likely mediated by NF-кB.