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目的:建立同时测定不同产地手掌参药材中多指标成分的质量控制方法,分析气候因子对手掌参分布活性成分含量的影响。方法:采用HPLC,Kromasil C18色谱柱(4.6 mm×250 mm,5μm),以0.1%醋酸水溶液和甲醇为流动相梯度洗脱,流速1.0 mL·min-1,检测波长270 nm,柱温30℃。结果:4种有效成分的线性关系均良好,精密度、稳定性和重复性的RSD均<2.50%,平均加样回收率为96.97%~101.55%,RSD均<1.46%;同时使用Diva-Gis提取不同产地手掌参药材的19个气候数据和3个地理数据进行聚类分析,经与样品有效成分含量测定的计量化学结果比较,表明不同产地手掌参药材各指标成分含量差异较大,通过气候因子的主成分分析和聚类分析,发现其与手掌参的活性成分的含量具有一定的相关性。结论:该方法准确,重复性好,可为科学、全面评价手掌参药材的品质提供参考,对进一步研究手掌参资源的可持续利用具有指导意义。
OBJECTIVE: To establish a method for the simultaneous determination of multi-index components in Radix Panacis Paellae from different areas, and to analyze the influence of climatic factors on the distribution of the active components of Radix Paeoniae. Methods: The mobile phase was eluted with a gradient of 0.1% acetic acid in water and methanol at a flow rate of 1.0 mL · min-1 using Kromasil C18 column (4.6 mm × 250 mm, 5 μm) with a detection wavelength of 270 nm and a column temperature of 30 ℃ . Results: The four active ingredients had good linearity, RSD <2.50%, average recovery of 96.97% ~ 101.55% and RSD <1.46% for precision, stability and repeatability. Nineteen climatic data and three geographical data were extracted from different regions of Panax notoginseng for clustering analysis. Comparing with the measurement results of the active ingredients of the samples, the results showed that the content of each index of the fingerprints of Panax notoginseng was significantly different, Factor analysis of the principal component analysis and cluster analysis found that the content of the active ingredient with the palm has a certain correlation. Conclusion: The method is accurate and reproducible. It can provide a scientific and comprehensive reference for evaluating the quality of Radix Paeoniae Alba, and it is instructive to further study on the sustainable utilization of Radix.