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为探讨不同疗程亚低温对新生儿脑损伤的保护作用,建立新生儿缺血缺氧性脑损伤亚低温干预大鼠模型.先在7日龄清洁级SD大鼠上以右颈总动脉结扎术和吸入低氧浓度气体法制备缺血缺氧性脑损伤(HIBD)模型,再将其随机分为室温恢复组(ROT组,N＝44)和3种疗程(24H,48H,72H)的亚低温干预组(HY24组,N＝26;HY48组,N＝14;HY72组,N＝20).亚低温干预期间采用配方奶喂养,并监测脑温、血糖、血气和血浆乳酸水平.实验2周后以体重增加百分率(WIP)、脑组织病变情况(右侧脑损伤分数和右侧海马细胞死亡率)判定疗效.结果,配方奶喂养的亚低温干预组的血糖维持在正常范围内(＞3MMOL/L),脑温降低4~5℃;HIBD后各组均出现酸中毒,HIBD后6HROT组的血气和血乳酸水平仍明显增高,而亚低温组已恢复正常.实验2周后HY24组的WIP明显高于ROT组(分别为134.5％、88.0％,P＜0.01),HY48与ROT组之间无明显差异(P＞0.05).病理学检查结果显示24~72H的亚低温干预使脑损伤程度降低了53％~89％,HY72组疗效最佳.这表明新生儿HIBD亚低温干预大鼠模型已成功建立,HIBD后“,”To establish the models of moderate hypothermia intervention after hypoxi cischemic brain damage (HIBD) in newborn rats,the posthypoxicischemi c 7dayold rats,weighing 14g±2g,were divided into 4 groups.The rats in gro up 1～3 (i.e.Hy24 group,n=26;Hy48 group,n=14;Hy72 group, n=20) were placed in water bath stabilized at 29℃ for 24 h,48 h,and 72 h respectively and fed with formular milk at the same time,and those in grou p 4 (RoT group,n=44) were stayed in a breeding room (25～29℃) with dams.After the insult,t...