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目的优选虎杖-桂枝药对的最佳醇提工艺。方法采用正交设计-重复试验法,以多波长HPLC法为含量测定方法,以出膏率、虎杖苷、桂皮醛、大黄素的含量为综合评价指标成分,优化提取工艺。结果优选的最佳提取工艺为用10倍量65%乙醇,热回流提取2次,每次60 min。结论该提取工艺简便、稳定性好、提取率高,可用于虎杖-桂枝药对有效成分提取。
Objective Optimum Polygonum cuspidatum - the best alcohol extraction process. Methods Orthogonal design - repeated test method, multi-wavelength HPLC method for the determination of content, the rate of the paste, polydatin, cinnamic aldehyde, emodin content of the comprehensive evaluation of the index components, optimize the extraction process. Results The optimal extraction process was as follows: extracting twice with 60% ethanol by refluxing with 10 times amount of 65% ethanol. Conclusion The extraction process is simple, good stability, high extraction rate, available for Polygonum cuspidatum - Guizhi drug extraction of active ingredients.