①目的构建人类巨细胞病毒ＡＤ１６９株即刻早期基因ＵＬ３７×１基因片段的原核表达克隆。②方法通过聚合酶链反应（ＰＣＲ）扩增目的基因，然后用限制性内切酶将目的基因与原核表达载体ｐＢＶ２２０定向连接。③结果获得了重组的原核表达质粒。④结论用限制性内切酶鉴定原核重组表达质粒构建成功 Objective To construct a prokaryotic expression clone of the immediate early gene UL37 × 1 gene of human cytomegalovirus AD169 strain. Methods The target gene was amplified by polymerase chain reaction (PCR), and then the target gene was ligated with the prokaryotic expression vector pBV220 by restriction enzyme. ③ Results obtained recombinant prokaryotic expression plasmid. ④ Conclusion Prokaryotic expression plasmid was successfully constructed by restriction endonuclease