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比较了休哈塔假丝酵母NLP21、树干毕赤酵母NLP22、NLP23和NLP31,在30g/L的木糖和混合糖(葡萄糖15g/L+木糖15g/L)发酵培养基上以及在培养基中氮源浓度降低到原来1/2和1/10时的发酵性能。结果表明,在30g/L木糖发酵培养基上,NLP23和NLP31产乙醇质量浓度最高,分别为(11.14±0.13)和(11.15±0.08)g/L。在15g/L葡萄糖+15g/L木糖混合糖发酵培养基上,NLP31产乙醇质量浓度最高,为(10.91±0.12)g/L。当发酵培养基中氮源浓度降低到原来的1/2时,NLP23和NLP31产乙醇能力相当,但后者产木糖醇的量增大;当氮源质量浓度降低到原来的1/10时,NLP23和NLP31产乙醇能力随着发酵轮数的增加,逐渐下降,氮源浓度低,降低了乙醇的产量。
Candida shehatae NLP21, Pichia stipitis NLP22, NLP23, and NLP31 were compared in fermentation broths of 30 g / L xylose and mixed sugars (glucose 15 g / L + xylose 15 g / L) and in medium The nitrogen source concentration was reduced to 1/2 and 1/10 of the original fermentation performance. The results showed that the ethanol concentration of NLP23 and NLP31 were the highest (11.14 ± 0.13) and (11.15 ± 0.08) g / L on the 30g / L xylose fermentation medium. The ethanol concentration of NLP31 was the highest (10.91 ± 0.12) g / L on 15g / L glucose + 15g / L xylose mixed sugar fermentation medium. When the concentration of nitrogen source in the fermentation medium was reduced to 1/2, the ethanol production capacity of NLP23 and NLP31 was quite high, but the amount of xylitol in the latter increased. When the concentration of nitrogen source was reduced to 1/10 The ethanol production capacity of NLP23 and NLP31 decreased with the number of fermentation cycles increasing, and the concentration of nitrogen source was low, which reduced the production of ethanol.