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蘑菇的多孢分离,大多沿用玻璃钟罩、培养皿、搪瓷盘等收集孢子的方法,操作复杂,不易掌握。我所采用试管收集孢子,操作比较简易,成功率也较高,现将其作法简介于下. (一)准备工作:①采收长至八分成熟的种菇(菌膜未破),用利刀将菇柄切去(留0.5cm左右),用消毒药水擦洗整个菇的表面。②取口径15mm的薄壁试管塞上棉塞,在1.5公斤压力下灭菌30分钟,也可将试管在酒精灯火焰上来回灼烧灭菌,然后棉塞过酒精灯火焰灭菌即塞上试管备用.③接种室或接种箱,用常规方法灭菌。
Spore separation of mushrooms, most of them follow the method of collecting spores in glass bell jar, petri dish, enamel dish and the like, and the operation is complicated and difficult to grasp. I used to collect spores test tube, the operation is relatively simple, the success rate is also higher, now its practice is briefly below (1) preparation: ① harvested as long as eight mature mushrooms (membrane is not broken), with Razor knife will be cut off (leaving 0.5cm or so), with a disinfectant scrub the entire surface of mushrooms. ② take the diameter of 15mm thin-walled tube stuffed cotton tampon, sterilized at 1.5 kg pressure for 30 minutes, the tube can also burn back and forth on the alcohol lamp flame sterilization, and then tampon over alcohol lamp flame sterilization plug Test tube spare ③ Inoculation room or inoculation box, sterilized by the conventional method.