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以异硫氰酸荧光素(FITC)标记沙拉沙星合成荧光标记物,采用薄层色谱法提纯,优化了反应时间、标记物和抗体的工作浓度,建立了沙拉沙星的快速荧光偏振免疫分析法(FPIA)。本方法测定沙拉沙星在缓冲液中的半数抑制浓度(IC50)为43.2μg/L;检测范围为5.7～327μg/L,可以达到国家规定的动物性食品中兽药最高残留限量(80μg/kg)的要求。本研究考察了FPIA测定沙拉沙星的动力学过程及对其它4种喹诺酮类药物的交叉反应。结果表明,环丙沙星、恩诺沙星、加替沙星及氧氟沙星的交叉反应率分别为3.3%,1.8%,1.7%和0.7%。在牛奶和猪尿中沙拉沙星的回收率分别在71%～94%和74%～102%之间。本方法操作简单快捷,整个检测过程只需5 min、而且灵敏度较高、特异性强,适用于动物性食品中沙拉沙星残留的快速筛选检测。
Fluorescein labeled with sarafloxacin was labeled with fluorescein isothiocyanate (FITC), purified by thin layer chromatography (TLC), and optimized the working concentrations of the reaction time, the marker and the antibody, and established a rapid fluorescence polarization immunoassay Act (FPIA). The IC50 value of sarafloxacin in the buffer was 43.2μg / L and the detection range was 5.7 ~ 327μg / L, which could reach the maximum residue limit of veterinary drugs (80μg / kg) Request. In this study, the kinetics of sarafloxacin determination by FPIA and cross-reactivity to four other quinolones were investigated. The results showed that the cross-reaction rates of ciprofloxacin, enrofloxacin, gatifloxacin and ofloxacin were 3.3%, 1.8%, 1.7% and 0.7% respectively. The recoveries of sarafloxacin in milk and pig urine ranged from 71% to 94% and from 74% to 102%, respectively. The method has the advantages of simple and quick operation, only 5 min in the whole testing process, high sensitivity and strong specificity, and is suitable for rapid screening and detection of sarafloxacin residues in animal foods.