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目的探索并建立一种基于循环micro RNA的诊断大鼠溃疡性结肠炎(ulcerative colitis,UC)方法并监测其治疗后效果的临床前策略。方法第1阶段:硫酸葡聚糖(Dextran sulfate sodium,DSS)诱导大鼠UC模型。实时定量RT-PCR筛选差异性的血清micro RNA。ELISA检测血清核周抗中性粒细胞胞浆抗体(periunclear antineutrophil cytoplasmic antibodies,p ANCA)。Logistic回归法建立诊断模型Indice。受试者工作特征(receiver operating characteristic,ROC)曲线评价指标的诊断效能。第2阶段:验证指标对UC的诊断效能,并用其评价UC的疗效。结果第1阶段:大鼠UC模型建立成功。Mi R-20b、mi R-21和let-7e*在UC大鼠血清中高表达,mi R-125b-1*无明显变化。p ANCA在UC大鼠血清中高表达。诊断模型Indice=0+(2.645×mi R-20b)+(1.622×mi R21)+(0.979×let-7e*)+(0.082×p ANCA)的诊断效能强于各单项指标。第2阶段:各指标均能监测UC疗效,Indice的监测效能最强。结论循环micro RNA能稳定高效的诊断UC并监测其治疗后效果。
Objective To explore and establish a preclinical strategy based on circulating micro RNA in the diagnosis of ulcerative colitis (UC) in rats and to monitor its effect after treatment. Methods Stage 1: Dextran sulfate sodium (DSS) -induced UC model in rats. Real-time quantitative RT-PCR screening of differentiated serum micro RNA. Serum perinuclear antineutrophil cytoplasmic antibodies (p ANCA) were detected by ELISA. Logistic Regression Method to Establish Diagnostic Model. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficacy of the indicator. Stage 2: To verify the diagnostic efficacy of indicators on UC and to evaluate the efficacy of UC. Results Stage 1: Rat UC model was established successfully. Mi R-20b, mi R-21 and let-7e * were overexpressed in the serum of UC rats, showing no significant change in mi R-125b-1 *. p ANCA is highly expressed in the serum of UC rats. Diagnostic model Indice = 0 + (2.645 × mi R-20b) + (1.622 × mi R21) + (0.979 × let-7e *) + (0.082 × p ANCA) had better diagnostic efficacy than individual indicators. Stage 2: All indicators can monitor the efficacy of UC, Indice monitoring the strongest. Conclusion Circulating microRNA can stably and efficiently diagnose UC and monitor its post-treatment effect.