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粉棒束孢是一种重要的昆虫病原真菌.运用ISSR分子标记研究了安徽省6个不同地理环境的粉棒束孢种群遗传异质性.结果表明:10个多态性引物多态位点百分率高达98.5%,但种群水平的多态位点差异较大,在59.6%～93.2%.基于Nei遗传异质性分析得出各种群间的遗传分化系数(Gst)为0.3365,基因流(Nm)为0.4931;各种群间的遗传分化低于种群内的遗传分化,表明安徽省粉棒束孢的遗传变异主要存在于种群内.根据各菌株间的遗传相似系数进行UPGMA聚类,结果表明,西山种群是单系的同质种群,其余5个种群皆为多系的异质种群,其中鹞落坪种群的异质性最高,琅琊山种群异质性最低.各种群之间的地理距离与遗传距离之间无相关关系.根据6个种群间的遗传距离进行UPGMA聚类,可将它们分成3个类群,分类的结果与各种群的地理环境相符,反映出环境的异质性对种群异质性的影响.
The genus Asclepiadia is an important insect pathogenic fungus.The ISSR molecular marker was used to study the genetic heterogeneity of the population of six populations in Anhui Province.The results showed that 10 polymorphic loci The percentage of polymorphic loci varied significantly from 59.6% to 93.2% at the population level, and the genetic differentiation coefficient (Gst) was 0.3365 based on Nei genetic heterogeneity analysis, and the gene flow ( Nm) was 0.4931, and the genetic differentiation among the populations was lower than that within the population, indicating that the genetic variation of Corydalis sp. In Anhui mainly existed in the population.According to the UPGMA clustering based on the genetic similarity coefficient among the isolates, The results showed that the Xishan population is a single-line homogeneous population, and the remaining five populations are heterogeneous populations of multi-lineages, of which the heterogeneity of the Yaoluoping population is the highest and that of the Langya mountain is the lowest There is no correlation between geographical distance and genetic distance.Using UPGMA clustering based on the genetic distance between 6 populations, they can be divided into 3 groups, the results of which are consistent with the geographical environment of each group, which reflect the heterogeneity of the environment The effect of sex on population heterogeneity.