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目的:研究万花油活络散瘀的作用。方法:①观察万花油对“血瘀证”大鼠耳廓微循环的影响,选取大鼠60只随机分为正常组、模型组、万花油低、中、高剂量组及麝香舒活精组,每组10只。对照组涂抹等量生理盐水,每日涂抹2次(间隔4 h),每次用量2 mL,连续3 d,给药结束记录耳廓微循环灌注量。②观察对外伤致小鼠皮下血瘀斑的影响,选取NIH小鼠50只,随机分为对照组、万花油低、中、高剂量组及麝香舒活精组,每组10只,每日涂抹2次连续涂药并观察4 d。每天观察并计算皮下瘀斑积分,比较各组差异。③观察对家兔软组织损伤的影响,选取新西兰兔36只,随机分为正常对组、模型组、万花油低、中、高剂量组及麝香舒活精组,每组6只。每日涂抹2次(间隔4 h),每次用量3 mL,连续7 d。末次给药1 h后,观察软组织损伤情况并做损伤等级评分;用酸度计测定打击中心部位肌组织pH值。结果:①与模型组灌流量(PU)80.27±14.32相比,万花油低、中、高剂量能明显促进“血瘀证”大鼠耳廓微循环,灌流量(PU)分别为:115.89±34.48,122.02±24.12,137.67±30.03(P<0.01,P<0.05);②与模型组相比,万花油低、中、高剂量在第2,3,4 d能明显降低外伤致小鼠皮下血瘀斑的作用(P<0.01,P<0.05);③与模型组相比,万花油能明显降低兔软组织的损伤(P<0.01,P<0.05)。结论:万花油有活络散瘀的作用。
Objective: To study the role of Wanhua oil activating stasis. Methods: ① The effect of Wanhua oil on auricle microcirculation in rats with “blood stasis syndrome” was observed. Sixty rats were randomly divided into normal group, model group, low, middle and high dose of Wanhua oil and musk Shu Huo Jing group, each group of 10. The control group was given the same amount of normal saline, smeared twice a day (interval 4 h), each dose of 2 mL, for 3 d, the end of recording the auricle microcirculation perfusion. ② Observe the effect of subcutaneous blood ecchymosis on mice induced by trauma. Fifty NIH mice were randomly divided into control group, low, middle and high dose of Wanhua oil and musk Shu Huo Jing Jing group Apply 2 times daily and observe for 4 days. Daily observation and calculation of subcutaneous ecchymosis score, comparing the differences between groups. (3) To observe the effect on soft tissue injury in rabbits. Thirty-six New Zealand rabbits were selected and randomly divided into normal group, model group, low, medium and high dose of Wanhua oil and musk Shu Huo Jing. Apply 2 times a day (4 h intervals) with 3 mL each for 7 days. One hour after the last administration, the injury of soft tissue was observed and the injury grade was scored. The acidity meter was used to measure the pH value of muscle tissue in the center. Results: (1) Compared with the model group (80.27 ± 14.32), the low, medium and high doses of Wanhua oil could obviously promote the auricle microcirculation in rats with “blood stasis syndrome” and the perfusion rate : 115.89 ± 34.48,122.02 ± 24.12,137.67 ± 30.03 (P <0.01, P <0.05). Compared with the model group, the low, medium and high doses of Wanhua oil could reduce trauma (P <0.01, P <0.05). ③ Compared with the model group, Wanhua oil could significantly reduce the soft tissue injury (P <0.01, P <0.05). Conclusion: Wanhua oil has an active stasis effect.