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[目的]研究不同浓度的桂皮醛(CA)对滑膜成纤维细胞(FLS)的增殖、凋亡及细胞周期的影响,进而探讨CA对骨关节炎滑膜炎症的调控机制。[方法]不同浓度的CA分别作用于LPS诱导后的FLS,通过四甲基偶氮唑盐微量酶反应比色法(MTT)、流式细胞术(FCM)对其增殖、凋亡和周期进行测定。[结果]低浓度的CA可轻度促进FLS的生长,较高浓度CA则可显著降低FLS的存活率(P<0.05),呈浓度依赖性。LPS可减少FLS的凋亡率,缩短G1期、延长S期,可用来制备炎症模型。与对照组相比,CA高浓度组与低浓度组均能是Q2和Q4区细胞比率明显增加(P<0.05),延长G1期并缩短S期(P<0.05),诱导FLS凋亡及抑制FLS的增殖,尤其是CA高浓度组作用更加显著。[结论]不同浓度的CA对FLS的生长均有一定的抑制作用,其中CA高浓度组的抑制作用更强,这可能是CA抗炎作用的潜在机制。
[Objective] To investigate the effects of different concentrations of cinnamic aldehyde (CA) on proliferation, apoptosis and cell cycle of synovial fibroblasts (FLS), and to explore the regulatory mechanism of CA on synovitis of osteoarthritis. [Method] Different concentrations of CA were applied to FLS induced by LPS respectively. The proliferation, apoptosis and cycle of FLS induced by LPS were detected by MTT assay and flow cytometry (FCM) Determination. [Result] Low concentration of CA could slightly promote the growth of FLS. Higher concentration of CA could significantly reduce the survival rate of FLS (P <0.05) in a concentration-dependent manner. LPS can reduce the apoptosis rate of FLS, shorten the G1 phase, prolong S phase, can be used to prepare the inflammation model. Compared with the control group, both CA high concentration group and low concentration group could significantly increase the cell ratio of Q2 and Q4 (P <0.05), prolong the G1 phase and shorten the S phase (P <0.05), and induce apoptosis and inhibition of FLS FLS proliferation, especially CA high concentration group effect is more significant. [Conclusion] Different concentrations of CA can inhibit the growth of FLS to a certain extent, and the inhibitory effect of CA in high concentration group is stronger, which may be the underlying mechanism of anti-inflammatory effect of CA.