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报道了用2.5Gy的~(60)Coγ射线照射外周血淋巴细胞,在照射后用间接免疫荧光法,检测单克隆抗体OKT_3~+、OKT_4~+和OKT_8~+与淋巴细胞膜抗原特异性结合的能力,以评价外周血T淋巴细胞亚群总T细胞(OKT_3)、辅助T细胞(OKT_4)和抑制T细胞(OKT_8)的变化。然后加白细胞介素-2(IL-2),观察IL-2对辐射损伤T淋巴细胞亚群的逆转效应。结果表明,T细胞亚群受2.5Gy~(60)Coγ射线照射后,照射组与不照射组相比,OKT_3~+、OKT_4~+和OKT_8~+细胞数均明显减少,且OKT_4/OKT_8的比例升高,这说明OKT_8细胞减少最多。当加入IL-2继续培养96h后与在同样条件下不加入(IL-2)的平行样本相比,其各群体的细胞荧光标记阳性百分率均明显增加。实验结果发现,IL-2可以使辐射损伤淋巴细胞的膜抗原恢复,对淋巴细胞的群体进行调节,使T淋巴细胞各亚群的细胞数目明显增加。
The peripheral blood lymphocytes were irradiated with 2.5 Gy of 60 Co gamma radiation. After inoculation, indirect immunofluorescence was used to detect the specific binding of the monoclonal antibodies OKT_3~+, OKT_4~+, and OKT_8~+ to the lymphocyte membrane antigen. Ability to evaluate changes in peripheral blood T-lymphocyte subsets total T cells (OKT_3), helper T cells (OKT_4) and suppressor T cells (OKT_8). Interleukin-2 (IL-2) was then added to observe the reversal effect of IL-2 on radiation-damaged T lymphocyte subsets. The results showed that the number of OKT_3~+, OKT_4~+, and OKT_8~+ cells in the irradiated group was significantly lower than that in the non-irradiated group after exposure to 2.5Gy~(60)Co γ-rays, and the number of OKT_4/OKT_8 The increase in the proportion indicates that OKT_8 cells have the greatest reduction. When IL-2 was added for 96 hours, the percentage of positive cells in each group was significantly increased compared with the parallel sample without IL-2 in the same conditions. The experimental results show that IL-2 can restore the membrane antigens of radiation-damaged lymphocytes and regulate the population of lymphocytes so that the number of cells in the subpopulations of T lymphocytes increases significantly.