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作者观察了LPS(脂多糖)对大鼠血清及内脏组织一氧化氮(NO)代谢产物NO_(2-)/NO_(3-)水平的影响。结果表明给于LPS后,血清 NO_2~-/NO_3~-水平急剧升高,24h达峰值,为对照组的20倍。心、肺组织的NO_2~-/NO_3~-含量也显著增加;但肝脏和肾脏组织NO_2~-/NO_3~-仅在短时期内增加。同时血清和内脏组织cGMP水平明显升高。给于NO合成酶(NOS)抑制剂L-NMMA,能降低血清和心、肺组织NO_2~-/NO_3~-升高幅度,并能减轻组织水肿程度。提示LPS能通过诱导NOS活性而增加体内NO水平,过多的NO以及cGMP可能是内毒素性组织的重要因素之一。
The authors observed the effect of lipopolysaccharide (LPS) on nitric oxide (NO) metabolites NO 2 - and NO 3 - in rat serum and visceral tissues. The results showed that the levels of NO 2 - / NO 3 - in serum increased sharply after LPS administration, reached the peak value at 24 h, which was 20 times of the control. NO_2 ~ - / NO_3 ~ - content in heart and lung tissues also increased significantly, but NO_2 ~ - / NO_3 ~ in liver and kidney increased only in a short period of time. Serum and visceral tissue cGMP levels were significantly higher. L-NMMA, an inhibitor of NO synthase (NOS), could reduce the increase of NO 2 - / NO 3 - in serum, heart and lung tissue and alleviate the degree of tissue edema. It is suggested that LPS can increase NO level by inducing NOS activity. Excessive NO and cGMP may be one of the important factors of endotoxin.