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用溴化氰活化右旋糖酐（Ｔ－７７）后，通过６－氨基己酸间隔桥，用水溶性碳二亚胺（ＥＤＣ）法将抗癌药甲胺喋呤（ＭＴＸ）与糖基相偶联，合成ＭＴＸ－右旋糖酐偶联物（ＭＴＸ－Ｄ）。应用细胞凋亡荧光染色（ＡＦＳ）法检测该偶联物ＭＴＸ－Ｄ 对人白血癌Ｋ５６２ 细胞的敏感性，结果该偶联物对Ｋ５６２ 细胞凋亡有诱导作用和影响其生长周期的作用。
After activating dextran (T-77) with cyanogen bromide, the anti-cancer drug methotrexate (MTX) was coupled to the glycosyl group via a 6-aminocaproic acid spacer bridge using a water soluble carbodiimide (EDC) MTX-dextran conjugates were synthesized (MTX-D). The sensitivity of the conjugate MTX-D to human leukemia K562 cells was detected by fluorescence microscopy (AFS). The results showed that the conjugate could induce the apoptosis of K562 cells and affect its growth cycle.