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本文采用分光光度计测定了山柰中总黄酮的含量,用甲醇为溶剂,回流提取4小时,以山柰酚作为对照品,在波长365nm处进行测定。方法简便、灵敏度高。
In this paper, the content of total flavonoids in Fructus Crataegi was determined by spectrophotometer. Methanol was used as the solvent and reflux extraction was carried out for 4 hours. Hawthorn was used as the reference substance and the determination was performed at the wavelength of 365 nm. The method is simple and high in sensitivity.