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目的 : 研究 Se和 VE对白血病细胞凋亡及 c- Myc基因表达的影响。方法 : 体外培养白血病细胞株 HL- 6 0、K5 6 2 ,培养时分别添加 Se(8μmol/L)和 VE(1 0 0 μmol/L)。利用 DNA凝胶电泳技术及 Northern杂交技术检测细胞凋亡和癌基因表达。结果 : Se和 VE均能诱导白血病细胞 HL- 6 0和 K5 6 2细胞凋亡。VE(1 0 0 μmol/L)能够抑制 HL- 6 0细胞中 c- Myc基因的表达 ,结果有显著性 ;VE却不能抑制 K5 6 2细胞内 c- Myc基因的表达。相反 ,Se(8μmol/L)不能够抑制 HL-6 0细胞中 c- Myc基因的表达 ,却能抑制 K5 6 2细胞内 c- Myc基因的表达 ,结果有显著性。结论 : 抑制 c- Myc基因的表达 ,诱导白血病细胞凋亡是 Se、VE抑制白血病细胞增殖的重要途径之一。对于髓系和非髓系白血病细胞 ,Se与 VE对 c- Myc基因的表达的影响迥然不同 ,提示二者的作用机制是不同的。
Objective : To study the effects of Se and VE on leukemia cell apoptosis and c-Myc gene expression. METHODS: The leukemic cell lines HL-60 and K562 were cultured in vitro. Se (8 μmol/L) and VE (100 μmol/L) were added during culture. DNA agarose gel electrophoresis and Northern blotting were used to detect apoptosis and oncogene expression. Results: Both Se and VE could induce apoptosis in leukemic cells HL-60 and K562 cells. VE (100 μmol/L) inhibited the expression of c-Myc gene in HL-60 cells, and the results were significant. VE could not inhibit the expression of c-Myc gene in K562 cells. On the contrary, Se (8 μmol/L) could not inhibit the expression of c-Myc gene in HL-6 0 cells, but it could inhibit the expression of c-Myc gene in K562 cells. The results were significant. Conclusion: Inhibiting the expression of c-Myc gene and inducing apoptosis of leukemia cells is one of the important ways for Se and VE to inhibit the proliferation of leukemia cells. For myeloid and non-myeloid leukemia cells, the effect of Se and VE on the expression of c-Myc gene is very different, suggesting that the mechanism of action of the two is different.