论文部分内容阅读
马铃薯晚疫病广谱抗性基因Rpi-blb1(克隆于Solanum bulbocastanum)及其两个同源抗病基因Rpi-sto1(克隆于Solanum stoloniferum)和Rpi-pta1(克隆于Solanum papita)的抗性可能已被高度进化的晚疫病菌株所克服。为证实此观点,将从荷兰田间和墨西哥野外S.bulbocastanum和S.stoloniferum病株上采集的晚疫病菌株重新接种于具有共同遗传背景(感病栽培品种Desiree)的Rpi-blb1及其同源基因转化体和3个野生种植株上,测试Rpi-blb1及其同源基因的抗性是否已被克服。结果表明,采自墨西哥野外S.stoloniferum的两个菌株Pic99189和Pic99192已经克服了Rpi-blb1及其同源基因的抗性;在野生种植株的测试中,只有S.papita的抗性明显地被菌株Pic99192所克服。同时发现在转基因植株离体叶片接种试验中,高抗晚疫病菌的转化体需要在多个转化体间进行选择。从采自中国4个不同地区晚疫病菌株接种sto1.Desiree转化体的小规模测试中可推断,Rpi-blb1及其同源抗性基因在中国仍具有潜在的应用价值。
The resistance of the broad-leaved potato late blight resistance gene Rpi-blb1 (cloned to Solanum bulbocastanum) and its two homologous resistance genes Rpi-sto1 (cloned to Solanum stoloniferum) and Rpi-pta1 (cloned to Solanum papita) It is overcome by highly pathogenic late blight strains. To confirm this view, late blight isolates collected from the S.bulbocastanum and S.stoloniferum isolates in the Dutch field and in Mexico were re-inoculated on Rpi-blb1 and its homologues with a common genetic background (susceptible cultivar Desiree) Transformants and 3 wild-type plants were tested for resistance to Rpi-blb1 and its homologues. The results showed that two strains Pic99189 and Pic99192 from S.stoloniferum in the Mexican field have overcome the resistance of Rpi-blb1 and its homologues; only S.papita resistance in wild-type plants was significantly Strain Pic99192 overcomes. At the same time, it was found that transformants with high resistance to P. infestans need to be selected among multiple transformants in in vitro leaf inoculation experiments in transgenic plants. In a small-scale test of sto1.Desiree transformants inoculated with P. infestans strains from 4 different areas in China, we concluded that Rpi-blb1 and its homologous genes still have potential applications in China.