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目的探讨宫颈病变组织中微小染色体维持蛋白7(MCM7)mRNA的表达及与HPV-16E7 mRNA的关系。方法采用半定量RT-PCR的方法检测2004年9月至2005年12月河北医科大学第二、第四医院102例宫颈组织中MCM7 mRNA和HPV-16E7 mRNA的表达。结果 (1)HPV-16E7 mRNA的表达水平在CINⅡ～Ⅲ及宫颈鳞癌明显高于慢性宫颈炎和CINⅠ(P<0.05);(2)MCM7 mRNA的表达水平在CINⅡ～Ⅲ及宫颈鳞癌明显高于慢性宫颈炎和CINⅠ,宫颈鳞癌明显高于CINⅡ～Ⅲ(P<0.05);(3)在CIN中,MCM7 mRNA在HPV16阳性组表达明显高于阴性组(P<0.01),在宫颈浸润癌中MCM7与HPV-16E7表达水平呈正相关(P<0.05);(4)在宫颈浸润癌中,MCM7 mRNA的表达随肿瘤分化程度增加而减少(P<0.05),而与年龄、临床分期无关。结论 HPV-16E7 mRNA的表达水平与宫颈病变的进展相关;MCM7 mRNA的表达与宫颈癌的发生、发展及与HPV-16感染密切相关。MCM7有望成为宫颈癌前病变筛查、监测生物学指标。
Objective To investigate the relationship between the expression of MCM7 mRNA and HPV-16E7 mRNA in cervical lesions. Methods The expression of MCM7 mRNA and HPV-16E7 mRNA in 102 cervical tissues from the second and fourth hospitals of Hebei Medical University from September 2004 to December 2005 were detected by semi-quantitative RT-PCR. Results (1) The expression of HPV-16E7 mRNA in CINⅡ ~ Ⅲ and cervical squamous cell carcinoma was significantly higher than that in chronic cervicitis and CINⅠ (P <0.05). (2) The expression of MCM7 mRNA was significantly higher in CINⅡ ~ Ⅲ and cervical squamous cell carcinoma (3) In CIN, the expression of MCM7 mRNA in HPV16-positive group was significantly higher than that in negative group (P <0.01). In cervical intraepithelial neoplasia There was a positive correlation between the expression of MCM7 and HPV-16E7 in invasive carcinoma (P <0.05). (4) The expression of MCM7 mRNA in invasive cervical carcinoma was decreased with the increase of tumor differentiation (P <0.05), but not with age, clinical stage Nothing to do Conclusion The expression of HPV-16E7 mRNA is correlated with the progression of cervical lesions. The expression of MCM7 mRNA is closely related to the occurrence and development of cervical cancer and HPV-16 infection. MCM7 is expected to become a precancerous cervical screening, monitoring of biological indicators.