论文部分内容阅读
本文对NK细胞与正常的,以及经低温、加热、超声、辐射、单糖、蛋白酶、和/或环己亚胺处理的靶细胞(AV、1C2、YAC-1、R.1.1,R.1.X)的相互作用进行了研究。并且与混合淋巴细胞培养产生的细胞毒作用进行了比较。试验并对来自L 5178 Y的一对变株AV(NK不敏感株)和1C2(NK敏感株)作了FACS分析。结果表明:NK细胞对多种瘤细胞均有杀伤作用,不受H-2限制,NK细胞介导的这一细胞毒作用可能需要一个完整的,有代谢活性的靶细胞结构。蛋白酶和环己亚胺处理能显著降低NK细胞的活性。FACS分析结果表明:1C2能特异地与抗Gg 3cer糖脂的单抗结合,而AV则不能。以上提示:NK细胞有它一定的识别作用。糖蛋白和/或糖脂可能是NK细胞所识别的靶的结构。
This article deals with NK cells and normal, as well as target cells treated with hypothermia, heating, ultrasound, radiation, monosaccharides, proteases, and/or cycloheximide (AV, 1C2, YAC-1, R.1.1, R.1 The interaction of .X) was studied. And compared with the cytotoxic effect produced by mixed lymphocyte culture. The FACS analysis was performed on a pair of strains AV (NK insensitive strains) and 1C2 (NK susceptible strains) from L 5178 Y. The results showed that NK cells have a killing effect on a variety of tumor cells and are not limited by H-2. NK cell-mediated cytotoxicity may require a complete, metabolically active target cell structure. Proteases and cycloheximide treatment significantly reduced the activity of NK cells. The results of FACS analysis showed that: 1C2 can specifically bind to the monoclonal antibody against Gg 3cer glycolipid, but AV can not. The above tips: NK cells have a certain degree of recognition. The glycoprotein and/or glycolipid may be the structure of the target recognized by NK cells.