根据苏云金芽胞杆菌杀虫晶体蛋白CrylA(b)和CrylA(c)的氨基酸序列,采用植物偏爱密码子,人工合成了全长1824bp的融合GFM CrylA Bt杀虫基因,并构建了带有多个表达调控元件的该基因高效植物表达载体pGBI1214AB,以及该基因和修饰豇豆胰蛋白酶抑制剂(CpTI)基因的双价杀虫基因植物表达载体pGBI1214ABC.经在烟草中验证,这两种表达载体在植物中可高效表达并赋予转基因烟草抗虫性.为我国抗虫棉的研究打下了基础. Based on the amino acid sequences of the insecticidal crystal proteins CrylA (b) and CrylA (c) of Bacillus thuringiensis, the full-length 1824bp fused GFM CrylA Bt insecticidal gene was constructed by using plant-preferred codons, And a bivalent insecticidal gene plant expression vector pGBI1214ABC containing the gene and a modified cowpea trypsin inhibitor (CpTI) gene, and the two expression vectors are verified in tobacco Which can be highly expressed and conferred resistance to transgenic tobacco and laid the foundation for the research on insect-resistant cotton in our country.