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本文采用药物梯度平皿筛选获得了耐利福定(Rifandin,RFD,R76-1)的大肠杆菌K-12菌株(RFD对耐药株的MICK对敏感株的125倍),利用同位素示踪技术对细菌耐RFD与细菌膜屏障的关系作了探讨。以3H-尿苷(3H-UR)掺入试验发现RFD或RFD合用膜非特异性通透剂EDTA均明显抑制敏感菌RNA合成(P<0.05);
In this paper, we obtained the Escherichia coli K-12 strain of Rifandin (RFD, R76-1) by drug gradient plate screening (RFD was 125 times more sensitive to MICK of the resistant strains), using isotope tracing technique Bacterial resistance to RFD and bacterial membrane barrier were discussed. The incorporation of 3H-Uridine (3H-UR) showed that both RFD and EDTA non-specific permeabilized EDTA significantly inhibited RNA synthesis (P <0.05).