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在大小麦感病品种的胚芽鞘大约长1—1.5cm 时接种白粉菌的分生孢子,置17—20℃的室温下20小时左右后,放入1—4℃的冰箱中保存。整个保存期间不加光照。30—40天后,胚芽鞘上长出白粉菌丝和分生孢子。经无病小麦苗接种试验,在3—4℃下小麦白粉菌保存110天、1—3℃下保存150天以上,胚芽鞘上的白粉菌分生孢子仍具有与盆栽麦苗上保存的分生孢子相似的侵染力。用胚芽鞘保存白粉菌种不需附加光照,方法简便,易于保持菌种纯度,保存期长。
When the coleoptile of large and small wheat susceptible species is about 1-1.5 cm in length, the conidial of the powdery mildew is inoculated and stored in a refrigerator at 1-4 ° C. for 20 hours at a temperature of 17-20 ° C. No light during the entire preservation period. After 30-40 days, the powdery mildew and conidia grow on the coleoptile. After the disease-free wheat seedling inoculation test, the wheat powdery mildew was stored at 3-4 ° C for 110 days and stored at 1-3 ° C for more than 150 days, the conidia of the powdery mildew in the coleoptile still had the same morphological characteristics as the meristem Spore-like infectivity. With the germ sheath save powdery mildew without additional light, the method is simple, easy to maintain the purity of the strain, long shelf life.