目的　研究同时作用于胰岛 β细胞第二分泌调控位点水平上的多种核苷酸代谢对胰岛素分泌量的影响。方法　以小鼠离体胰岛细胞为材料 ,用二氮嗪阻断 β细胞膜上的 K+ - ATP通道作用后 ,将细胞外液 K+浓度提高到30 mmol/ L ;用葡萄糖诱导胰岛素分泌 ,同时测定胰岛素分泌量、ATP、ADP、GTP、GDP和 UTP水平 ,并分析它们之间的关系。结果　在上述实验条件下 ,胰岛素分泌仍随葡萄糖浓度增加而增加 ,呈现正常的剂量相关关系 ;与此同时 ,ATP/ADP、GTP/ GDP和 UTP也同步升高 ,三者分别都与胰岛素分泌量呈高度的线性正相关关系 ,但在共同的作用中 ,只有ATP/ ADP能直接对胰岛素分泌产生影响。结论　葡萄糖诱导胰岛素分泌时 ,β细胞内存在第二个细胞调控点 ,ATP/ADP比率是这个调控点的决定因素 Objective To study the effects of multiple nucleotide metabolism on insulin secretion at the second secretion regulatory site of pancreatic β cells. Methods Mouse isolated islet cells were treated with diazoxide to block the K + - ATP channel on β - cell membrane, and the concentration of extracellular K + was increased to 30 mmol / L. Glucose was used to induce insulin secretion. Insulin Secretion, ATP, ADP, GTP, GDP and UTP levels, and analyze the relationship between them. Results Under the above experimental conditions, insulin secretion still increased with the increase of glucose concentration, showing a normal dose-related relationship; at the same time, ATP / ADP, GTP / GDP and UTP also increased simultaneously, the three were associated with insulin secretion A highly linear positive correlation, but in a common role, only ATP / ADP can directly affect insulin secretion. Conclusions When glucose induces insulin secretion, there is a second cell control point in β cells. The ATP / ADP ratio is the determinant of this regulation point