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DNA重组技术可用于生产传染性微生物的各种蛋白质,这些蛋白质能用于制备亚单位疫苗。然而,在许多情况下,这些蛋白质的免疫原性较低。原因之一可能是抗原向免疫系统细胞的提呈较差。目前,正在使用许多新的DNA重组方法来改善抗原产量和提呈,一种方法是使用由酵母逆转座子(Ty因子)编码的载体蛋白,这种蛋白能通过构建杂种基因与多种病毒抗原连接。这种融合蛋白在酵母中装配成60nm的病毒样颗粒(VLP),在VLP表面呈现附加抗原。这些杂种Ty-VLP即使在缺乏佐剂的情况下也是良好的免疫原。相似的生产多价颗粒抗原的方法包括使用来自乙型肝炎和脊髓灰质炎病毒的载体蛋白。
DNA recombinant technology can be used to produce a variety of infectious microorganisms, these proteins can be used to prepare subunit vaccines. However, in many cases these proteins are less immunogenic. One of the reasons may be poor antigen presentation to immune system cells. At present, many new DNA recombination methods are being used to improve antigen production and presentation. One method is to use a carrier protein encoded by yeast retrotransposon (Ty factor), which can be constructed by constructing hybrid genes with various viral antigens connection. This fusion protein is assembled in yeast into 60-nm viral-like particles (VLPs) that present additional antigens on the VLP surface. These hybrid Ty-VLPs are good immunogens even in the absence of adjuvant. Similar methods for producing multivalent particulate antigens include the use of carrier proteins from Hepatitis B and poliovirus.