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目的研究双酚A(Bisphenol A,BPA)对小鼠的氧化损伤作用,探讨中低剂量双酚A致小鼠肝肾氧化损伤机制。方法将60只4周龄昆明(KM)雄性小鼠按体重随机分为6组,分别予以不同剂量BPA(0.0004、0.004、0.04、10、50 mg/kg)玉米油溶液灌胃,每天染毒一次,连续染毒30 d。处死后取肝肾脏组织制备匀浆液,采用WST-1法测定超氧化物歧化酶(SOD)活性,用紫外分光法测定过氧化氢酶(CAT)活性,化学比色法测定谷胱甘肽过氧化物酶(GSH-Px)的活性,比色法测定丙二醛(MDA)的含量,以分光光度法测定羟自由基含量,用化学比色法测定过氧化氢以及超氧阴离子自由基的含量。结果与对照组比较,除0.0004 mg/kg剂量组肾O2-外,各剂量组自由基及丙二醛含量显著升高,且随染毒剂量的增加而升高,差异有统计学意义(P<0.05);而抗氧化酶活性,除0.0004 mg/kg剂量组肾SOD外,各剂量组肝、肾SOD、GSH-Px、CAT活性随染毒剂量增加而下降。结论中低剂量BPA明显致小鼠的肝肾抗氧化酶活性降低、MDA及氧自由基含量增加,从而导致氧化损伤。
Objective To study the effect of bisphenol A (BPA) on the oxidative injury in mice and explore the mechanism of oxidative damage induced by middle and low doses of bisphenol A in mice. Methods Sixty KM Kunming (KM) male mice, 4 weeks old, were randomly divided into 6 groups according to body weight. The rats were orally administered with different doses of BPA (0.0004, 0.004, 0.04, 10, 50 mg / Once, continuous exposure 30 d. After sacrificed, liver and kidney tissues were taken to prepare homogenate, the activity of superoxide dismutase (SOD) was measured by WST-1 method, catalase (CAT) activity was measured by ultraviolet spectrophotometry, glutathione The activity of GSH-Px was determined. The content of malondialdehyde (MDA) was determined by colorimetry. The content of hydroxyl radical was determined by spectrophotometry. The contents of hydrogen peroxide and superoxide anion radical content. Results Compared with the control group, the content of free radicals and malondialdehyde in each dose group was significantly increased except for 0.0002 mg / kg of renal O2-, and increased with the increase of exposure dose (P <0.05). The activity of SOD, GSH-Px and CAT in the liver and kidney of each dose group decreased with the increase of the dose of SOD except the activity of SOD in the dose of 0.0004 mg / kg. Conclusion Low and medium doses of BPA significantly reduced the antioxidant activity of liver and kidney, and the content of MDA and oxygen free radicals in mice increased, leading to oxidative damage.