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目的探讨新疆维吾尔族(维族)和汉族食管鳞状细胞癌(鳞癌)患者凋亡相关基因膜转运蛋白166(transmembrane protein 166,TMEM166)启动子区CpG岛甲基化的差异,及其与食管鳞癌临床病理特征及预后的关系。方法 40例食管鳞癌患者,其中汉族和维族各20例,采用甲基特异性PCR法检测癌组织和癌旁组织TMEM166启动子区CpG岛甲基化情况,分析TMEM166基因甲基化与食管鳞癌临床病理及预后的关系。结果癌组织TMEM166启动子区CpG岛甲基化阳性率(75.0%)明显高于癌旁组织(45.0%)(P<0.05);维族TMEM166启动子区CpG岛甲基化阳性率(70.0%)与汉族(80.0%)比较差异无统计学意义(P>0.05);食管鳞癌Ⅲ期患者TMEM166启动子区CpG岛甲基化阳性率(95.0%)明显高于Ⅱ期患者(51.0%)(P<0.05);食管鳞癌患者TMEM166启动子区CpG岛甲基化阳性率在民族、性别、年龄上差异无统计学意义(P>0.05);TMEM166启动子区CpG岛甲基化阳性者中位生存时间为28个月,阴性者为27个月,差异无统计学意义(P>0.05)。结论食管鳞癌组织TMEM166启动子区CpG岛甲基化水平高于癌旁组织,TMEM166启动子区CpG岛甲基化阳性率可能与食管鳞癌TNM分期有关,与患者年龄、性别、民族和预后无关。
Objective To investigate the difference of methylation of CpG island in promoter region of apoptosis-associated gene transmembrane protein 166 (TMEM166) between Uighur (Uighur) and Han esophageal squamous cell carcinoma (squamous cell carcinoma) Relationship between clinicopathological features and prognosis in squamous cell. Methods Forty esophageal squamous cell carcinoma patients, including 20 Han and Uygur nationals, were examined for the methylation of CpG island in TMEM166 promoter region by PCR using methyl-specific PCR. The methylation of TMEM166 gene and esophageal squamous cell carcinoma Relationship between clinicopathology and prognosis of cancer. Results The positive rate of methylation of CpG island in TMEM166 promoter region (75.0%) was significantly higher than that in adjacent non-cancerous tissues (45.0%) (P <0.05). The positive rate of methylation of CpG island in TMEM166 promoter region was 70.0% (95.0%) in TMEM166 promoter region was significantly higher than that in stage II patients (51.0%) (P> 0.05), but there was no significant difference between them (80.0% (P> 0.05). The positive rate of methylation of CpG island in TMEM166 promoter region in esophageal squamous cell carcinoma patients was not significantly different among nationalities, sex and age (P> 0.05). In TMEM166 promoter region, The median survival time was 28 months and the negative was 27 months, the difference was not statistically significant (P> 0.05). Conclusions The methylation level of CpG island in TMEM166 promoter region of esophageal squamous cell carcinoma is higher than that of paracancerous tissues. The positive rate of CpG island methylation in TMEM166 promoter region may be related to the TNM stage of esophageal squamous cell carcinoma and to the age, gender, ethnicity and prognosis Nothing to do