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目的:通过建立大鼠子宫内膜异位症(EM)模型,从EM模型异位病灶局部组织细胞凋亡的变化探讨石见穿对EM的作用。方法:建立EM动物模型,将建模成功大鼠随机分为非治疗组、石见穿治疗组、丙氨瑞林组,将正常大鼠设为正常对照组。通过TUNEL法检测各组大鼠在位及异位内膜组织细胞凋亡率。结果:模型组异位内膜、在位内膜组织细胞凋亡率均明显低于正常对照组,差异有显著性(P<0.05),而异位内膜细胞凋亡率低于在位内膜,差异有显著性(P<0.05);石见穿治疗组在位内膜、异位内膜细胞凋亡率均高于模型组,有显著性差异(P<0.05)。结论:石见穿治疗组异位内膜凋亡敏感性明显增强,且凋亡细胞分布广,其明显抑制异位内膜生长的作用可能是通过诱导异位内膜细胞凋亡的方式来实现的。
OBJECTIVE: To investigate the effect of SHI MEI-TONG on EM through the establishment of rat endometriosis (EM) model and the changes of local tissue cell apoptosis in ectopic lesions of EM model. METHODS: An animal model of EM was established. Successfully modeled rats were randomly divided into non-treatment group, Shiminchuan treatment group, and amirarolin group, and normal rats were used as normal control group. The TUNEL method was used to detect the apoptosis rate of eutopic and ectopic endometrium cells in each group. Results: The apoptotic rate of ectopic endometrium and eutopic endometrium in the model group was significantly lower than that in the normal control group (P<0.05), while the apoptotic rate of ectopic endometrial cells was lower than that in the control group. Membrane, the difference was significant (P <0.05); Shimijuan treatment group eutopic and ectopic endometrial apoptosis rate was higher than the model group, there was a significant difference (P <0.05). Conclusion: The susceptibility of ectopic endometrial apoptotic cells was significantly increased in Shimeichuan treatment group, and apoptotic cells were widely distributed. Its effect of inhibiting ectopic endometrial growth may be achieved by inducing ectopic endometrial apoptosis. of.