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目的 研究局部脑缺血模型小胶质细胞的激活和白细胞的浸润情况 ,以进一步研究脑卒中后引起炎症反应的细胞及分子机制。方法 用免疫组化方法研究单克隆抗体CD11b ,Ly 6G ,CD3和CD4 5 /B2 2 0在大脑中动脉闭塞 (MCAO) 6 0min进行不同时段灌注后在脑组织中的表达。结果 灌注早期 (18～ 2 4h)在脑梗塞区就可见被激活的CD11b阳性的小胶质细胞 ,在 18～ 2 4h灌注期 ,可见CD11b阳性的巨噬细胞和Ly 6G阳性的中性白细胞黏附在脑膜的血管壁。灌流 4 8～ 72h ,CD11b阳性的巨噬细胞和Ly 6G阳性的中性白细胞从血管壁迁移到脑梗塞区。在灌流 2 4～ 4 8h ,我们仅见到个别CD3阳性T淋巴细胞和CD4 5 /B2 2 0阳性B淋巴细胞。然而 ,在灌流 72h后 ,可容易地检测到CD3阳性T淋巴细胞。 结论 研究结果表明单核吞噬细胞系统在MCAO脑卒中模型的炎症反应中起重要作用。在灌注早期CD11b阳性细胞主要为脑实质内被激活的小胶质细胞 ,灌注 2 4～ 72h ,外周血循环中的巨噬细胞和其他白细胞从血管迁移到缺血区以及被激活的小胶质细胞一同在脑卒中炎症反应中起作用。
Objective To study the activation of microglia and the infiltration of leukocytes in focal cerebral ischemia model in order to further investigate the cellular and molecular mechanism of inflammatory response after stroke. Methods Immunohistochemistry was used to investigate the expression of CD11b, Ly 6G, CD3 and CD4 5 / B2 2 0 in brain after perfusion of MCAO for 60 min. Results In the early stage of perfusion (18 ~ 24 h), CD11b positive microglial cells were activated in cerebral infarction area. During the 18 ~ 24 h perfusion period, CD11b positive macrophages and Ly 6G positive neutrophils adhered The vessel wall in the meninges. 48 ~ 72h after perfusion, CD11b-positive macrophages and Ly 6G-positive neutrophils migrated from the vascular wall to the infarcted area. We only see individual CD3-positive T lymphocytes and CD4 5 / B2 2 0-positive B lymphocytes 24 to 48 h after perfusion. However, CD3-positive T lymphocytes were readily detectable after 72 h of perfusion. Conclusions The results suggest that the mononuclear phagocyte system plays an important role in the inflammatory response of MCAO stroke models. In the early perfusion of CD11b positive cells mainly in the brain parenchyma activated microglia, perfusion 24 ~ 72h, macrophages and other leukocytes in the peripheral blood circulation from the blood vessels to the ischemic area and activated microglia Work together in the stroke inflammatory response.