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本研究中制备了表达表皮生长因子受体(EGFR)部分肽段的基因重组T7噬菌体疫苗,并开展了诱导小鼠产生内源性抗EGFR抗体的实验性抗肿瘤作用研究。由T7噬菌体展示系统将7个经筛选的异种属(人源、鸡源)EGFR膜外区片段展示在其壳体次要头蛋白(P10B)上,用所制备的基因重组噬茵体疫苗免疫小鼠,免疫4W后皮下接种Lewis肺癌细胞,10d后分离瘤体并称重,观察各实验组的抗肿瘤效果。Western Blot检测重组的融合壳蛋白均有EGFR抗原性:高表达EGFR的A431 细胞与免疫3W的小鼠抗血清结合并被荧光二抗标记,流式细胞仪检测法确认有抗EGFR抗体产生;各实验组肿瘤均重统计结果显示,P-CL1-670组、P-cp1-130组、P-cp2-136组、P-cp3-145组、 P-cp4-142组与空白噬菌体组差异性显著。说明表达EGFR的基因重组噬菌体疫苗诱导产生的内源性抗体．在一定程度上抑制了EGFR阳性肿瘤的生长．为诱导型内源性抗EGFR抗体的肿瘤靶向治疗研究开辟了新的途径。
In this study, recombinant T7 phage vaccine expressing partial peptide of epidermal growth factor receptor (EGFR) was prepared and the experimental anti-tumor effect of inducing endogenous anti-EGFR antibody in mice was studied. Seven screened foreign gene fragments of human heterologous (human, chicken) EGFR were displayed on the shell podocyte protein (P10B) by the T7 phage display system and immunized with the prepared recombinant phage vaccine Mice were inoculated with Lewis lung cancer cells subcutaneously 4 weeks after immunization. After 10 days, the tumors were separated and weighed, and the anti-tumor effect of each experimental group was observed. Western Blot detection of recombinant fusion capsid protein has EGFR antigenicity: high expression of EGFR A431 cells and immune 3W mouse antiserum bound and labeled with a fluorescent secondary antibody, flow cytometry confirmed anti-EGFR antibody production; each The mean tumor weight in experimental group showed significant difference between P-CL1-670 group, P-cp1-130 group, P-cp2-136 group, P-cp3-145 group and P-cp4-142 group and blank phage group . Explanations of endogenous antibodies induced by recombinant phage vaccine expressing EGFR. To a certain extent, inhibited the growth of EGFR positive tumors. It has opened up a new way for the research of tumor targeting therapy of induced endogenous anti-EGFR antibody.