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目的 建立和评价能准确检测食管鳞癌 (ESC)局部淋巴结 (LN)微小转移的RT PCR法。方法 取 2 3例ESC患者的局部LN共 10 4枚 ,将每枚LN均分为两等份 ,分别行病理学检查和CK19mRNA的表达分析。采用连续稀释试验评价方法的灵敏度。结果 本法的灵敏度为 10 -6～10 -4 μgESCRNA 1μg正常LNRNA。 2 2枚LN被病检法发现存在转移 ,RT PCR法发现其中 2 1枚为阳性。在余下的 82枚病检阴性LN中 ,RT PCR还发现 10枚存在微转移。结论 RT PCR法检测局部LN转移有助于ESC患者亚临床转移的早期诊断和治疗。
Objective To establish and evaluate an RT PCR method capable of accurately detecting micrometastases of esophageal squamous cell carcinoma (ESC). Methods Totally 104 local LNs were obtained from 23 ESC patients. Each LN was divided into two equal parts and pathologically examined and CK19 mRNA expression was analyzed respectively. Continuous dilution test was used to evaluate the sensitivity of the method. Results The sensitivity of this method was 10 -6 ~ 10 -4 μg ESCRNA 1 μg normal LNRNA. Two LNs were found to be metastasized by the pathology test, and 21 of them were positive by RT PCR. Of the remaining 82 pathologically negative LNs, RT PCR also revealed the presence of 10 micrometastases. Conclusion The detection of local LN metastasis by RT-PCR is helpful for the early diagnosis and treatment of subclinical metastasis in ESC.