论文部分内容阅读
近年来,丙型肝炎病毒(hepatitis C virus,HCV)母婴传播已成为 HCV 传播途径研究中的一个热点。我们曾对受 HCV感染孕妇的引产胎儿肝组织内 HCV RNA 的定位研究,证实HCV 母婴传播途径的存在。本文通过对孕妇/胎儿、产妇/新生儿和婴儿中检出的 HCV 基因型别的鉴定、孕妇血/胎儿肝组织内的 HCV C 区基因片段核苷酸序列分析,从 HCV 基因型分布和基因序列同源程度,探讨 HCV 的母婴传播。资料与方法一、研究对象23对 HCV RNA 和抗-HCV 均阳性的产妇外周静脉血/新生儿脐带血以及不同月龄随访到的婴儿外周静脉血标本、4对 HCV RNA 阳性的孕妇外周静脉血/引产胎儿肝组织匀浆标本。二、HCV 基因型分析采用 Okamoto 等的 HCV C 区基因逆转录——巢式聚合酶链反应(RT-Nested PCR)分型方法与命名系统。三、HCV C 区基因片段的克隆与测序
In recent years, mother-to-infant transmission of hepatitis C virus (HCV) has become a hot spot in the study of HCV transmission. We have investigated the localization of HCV RNA in fetal liver tissue from HCV-infected pregnant women, confirming the existence of HCV maternal-to-infant transmission. In this paper, the HCV genotypes detected in pregnant women / fetuses, mothers / newborns and infants were analyzed. Sequence homology, to explore the mother-to-child transmission of HCV. MATERIALS AND METHODS A. Subjects 23 Peripheral venous blood / neonatal umbilical cord blood samples positive for both HCV RNA and anti-HCV and peripheral blood samples from infants who were followed up at different months of age were analyzed. Four peripheral blood samples of pregnant women with positive HCV RNA / Induction of fetal liver homogenate samples. HCV genotype analysis Okamoto et al. HCV RT-Nested PCR typing and nomenclature system were used. Third, HCV C region gene cloning and sequencing