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目的:从细胞水平探讨高压氧或(和)5-氟尿嘧啶(5-FU)处理对鼻咽癌CNE-2Z细胞增殖与侵袭转移的影响及其影响机制。方法:将实验用人鼻咽癌CNE2Z细胞分为对照组(A组)、5-FU组(B组)、高压氧组(C组)、5-FU加高压氧组(D组)4组。采用MTT比色法观察各组处理24、48和72h后对鼻咽癌CNE-2Z细胞增殖的抑制作用;采用Transwell小室分析法评价各组处理对鼻咽癌细胞侵袭、迁移相关能力的影响;采用细胞免疫化学染色法检测各组鼻咽癌细胞MMP-9、VEGF的表达水平。结果:C组处理48h和72h后鼻咽癌细胞增殖抑制A值与A组比较均差异有统计学意义(均P<0.01),D组处理仅48h后分别与A、B、C组比较差异有统计学意义(P<0.05);C组鼻咽癌细胞侵袭迁移结果和MMP-9和VEGF表达的平均值与A组比较差异均无统计学意义(P>0.05),虽D组鼻咽癌细胞侵袭、迁移结果和MMP-9和VEGF表达的平均A值与A组比较均差异有统计学意义(P<0.01),但与C组比较均差异无统计学意义(P>0.05)。结论:单纯高压氧处理48h和72h后均能明显抑制人鼻咽癌细胞的增殖,但高压氧和5-FU联合处理仅在处理48h后才能更明显抑制人鼻咽癌细胞的增殖;单纯高压氧处理不能明显降低鼻咽癌细胞MMP-9、VEGF的高表达,也不能抑制鼻咽癌细胞的侵袭迁移能力;并且高压氧联合5-FU处理也不能较单纯5-FU处理更进一步降低鼻咽癌细胞MMP-9、VEGF的表达和抑制鼻咽癌细胞侵袭与迁移。
OBJECTIVE: To investigate the effects of hyperbaric oxygen or 5-fluorouracil (5-FU) treatment on the proliferation, invasion and metastasis of CNE-2Z cells at the cellular level and its mechanism. Methods: CNE2Z cells were divided into four groups: control group (group A), 5-FU group (group B), hyperbaric oxygen group (group C) and 5-FU plus hyperbaric oxygen group (group D). MTT assay was used to observe the inhibitory effect of each group on the proliferation of CNE-2Z cells after treated for 24, 48 and 72 hours. The transwell assay was used to evaluate the effect of each treatment on the invasion and migration-related capacity of NPC cells. Immunocytochemical staining was used to detect the expression of MMP-9 and VEGF in each group of nasopharyngeal carcinoma cells. Results: The inhibitory value of proliferation inhibition of nasopharyngeal carcinoma cells in group C at 48h and 72h was significantly different from that in group A (all P <0.01). The difference between group A and group B was only 48h (P <0.05). There was no significant difference in the invasion and migration of MMP-9 and VEGF between group C and group A (P> 0.05). Although nasopharyngeal The invasion and migration of cancer cells and the mean A value of MMP-9 and VEGF expression were significantly different from those in group A (P <0.01), but there was no significant difference between group C and group C (P> 0.05). CONCLUSION: Both hyperbaric oxygen treatment and 5-FU treatment can significantly inhibit the proliferation of human nasopharyngeal carcinoma cells 48h and 72h after treatment with hyperbaric oxygen alone. Oxygen treatment can not significantly reduce the high expression of MMP-9 and VEGF in nasopharyngeal carcinoma cells, and also can not inhibit the invasion and migration of nasopharyngeal carcinoma cells; and hyperbaric oxygen combined with 5-FU treatment can not further reduce the nasal The expression of MMP-9 and VEGF in pharyngeal carcinoma cells and the inhibition of nasopharyngeal carcinoma cell invasion and migration.