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目的:探讨溶血标本对电化学发光法检测血清抗环瓜氨酸肽抗体(Anti-CCP)的干扰作用。方法:依据美国临床实验室标准化委员会制定EP7-P文件标准程序,取10例正常人新鲜血液分离血清及其红细胞,配对后配制成不同浓度的溶血模型,检测血红蛋白浓度与溶血指数之间的关系。分析溶血指数与电化学发光法检测血清Anti-CCP的关系,并且检测56例类风湿关节炎(RA)患者、35例其他风湿性疾病患者及30名健康对照者血清中的Anti-CCP,观察标本血清Anti-CCP检测结果的分布。结果:血红蛋白浓度与溶血指数在临床标本溶血范围内呈良好的线性关系,Y(溶血指数)=108.4X(血红蛋白浓度)-4.1,R2=0.990 6。24例正常人标本的不同溶血程度对Anti-CCP检测结果产生正干扰。RA患者血清Anti-CCP水平显著高于健康对照者,并且Anti-CCP临界值少见。结论:溶血干扰电化学发光法检测血清Anti-CCP结果,且Anti-CCP是观察判断RA患者一项较好的参考指标。
Objective: To investigate the interference effect of hemolysate on the detection of serum anti-cyclic citrullinated peptide antibody (Anti-CCP) by electrochemiluminescence. Methods: Based on the standard procedure of EP7-P document developed by the American Clinical Laboratory Standardization Committee, 10 normal individuals were divided into fresh blood to separate the serum and erythrocytes. After matching, hemolysis models were prepared to detect the relationship between hemoglobin concentration and hemolytic index . The relationship between hemolysis index and the detection of serum Anti-CCP by electrochemiluminescence was analyzed. Anti-CCP in sera of 56 rheumatoid arthritis (RA) patients, 35 rheumatic diseases patients and 30 healthy controls were detected. Distribution of serum anti-CCP test results. Results: There was a good linear relationship between hemoglobin concentration and hemolytic index in the hemolysis range of clinical samples. Y (hemolytic index) = 108.4X (hemoglobin concentration) -4.1, R2 = 0.990 6.24 different hemolysis degree of normal human specimens Anti The -CCP test results in positive interference. Serum anti-CCP levels in patients with RA were significantly higher than those in healthy controls, and the anti-CCP cutoff was rare. Conclusion: The results of serum anti-CCP by hemolysis interference electrochemiluminescence assay and Anti-CCP is a better reference index for judging RA patients.