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本文对60例各种急、慢性乙型肝炎患者外周血T淋巴细胞亚群和血清中IL-6及ALT水平进行检测.结果报告如下.1 材料和方法1.1 研究对象 20例急性乙肝患者,年龄26～44岁,血清中,HBsAg和HBV-DNA阳性,病程在半年以内.40例HBsAg阳性的慢性乙肝患者,病程半年以上,年龄28～5l岁,其中HBV-DNA阳性和阴性各20例.健康对照20例,年龄25～35岁,乙型肝炎(以下简称乙肝)和丙型肝炎血清标志均阴性.1.2 主要试剂 T细胞亚群采用APAAP法测定,试剂购自华美公司,IL-6采用ELISA法定量测定,试剂购自SIGMA公司,HBV-DNA用套式PCR法,试剂购自复旦大学.1.3 统计学处理 组间比较采用t检验,相关性采用线性相关检验.ALT水平显著高于对照组(P<
In this paper, 60 cases of various acute and chronic hepatitis B patients with peripheral blood T lymphocyte subsets and serum levels of IL-6 and ALT were reported.The results are reported as follows.1 Materials and Methods 1.1 Study Subjects 20 cases of acute hepatitis B patients, age 26 to 44 years old, serum HBsAg and HBV-DNA positive, course of disease within six months.40 cases of HBsAg-positive chronic hepatitis B patients, the course of more than six months, aged 28-51 years, including HBV-DNA positive and negative in 20 cases. 20 healthy controls, aged 25-35 years, hepatitis B (hereinafter referred to as hepatitis B) and hepatitis C serum markers were negative.1.2 The main reagent T cell subsets APAAP assay, reagents purchased from China and the United States, IL-6 ELISA assay, reagent purchased from SIGMA company, HBV-DNA by nested PCR method, reagent purchased from Fudan University .1.3 Statistical analysisComparison between groups using t test, correlation using linear correlation test.ALT level was significantly higher than the control Group (P <