采用高效液相色谱－间接光度检测法（ＨＰＬＣ－ＩＰＤ），在流动相中加入具有紫外检测响应的检测剂烟酰胺，用紫外检测器直接测定体液中无紫外吸收的西梭霉素含量。固定相为ＳｐｈｅｒｉｓｏｒｂＣ１８，流动相为甲醇－乙腈－水（２０∶１０∶７０），内含磷酸０．０５ｍｏｌ／Ｌ、烟酰胺０．５ｍｍｏｌ／Ｌ、庚烷磺酸钠５ｍｍｏｌ／Ｌ。血清样品中西梭霉素平均回收率为９６．９２％±４．６３％，日内和日间变异系数分别为４．７５％和５．６５％。 High-performance liquid chromatography-indirect photometric detection (HPLC-IPD) was used to add nicotinamide, a detector with a UV detection response, to the mobile phase. The UV absorbers were used to determine the concentration of sibycin without UV absorption. The stationary phase was Spherisorb C18. The mobile phase was methanol-acetonitrile-water (20:10:70) containing 0.05 mol / L phosphoric acid, 0.5 mmol / L nicotinamide and 5 mmol / L heptane sulfonate. The mean recoveries of sisamycin in serum samples were 96.92% ± 4.63%, and the intra-and inter-day coefficients of variation were 4.75% and 5.65%, respectively.