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Aiming at solving the existing issues in purity identification of Cucurbita moschata hybrids by SSR,such as complex operation and difficult application in production practice,in this study,a simple SSR-based method was established for purity identification of Cucurbita moschata hybrids. By using the established simple method,without grinding,freezing,centrifugation and drying,the genomic DNA extraction process is shorter than 3 min. Compared with the conventional method,PCR detection system and silver staining in polyacrylamide gel electrophoresis of the established method are more time-saving and cost-saving. The whole detection process is shorter than 4 h,and 480 samples can be detected with this method by one person in one day. In addition,the detection result exhibits a coincidence rate of 99% with field identification. The simple SSR-based method established in this study can provide basis for large-scale rapid purity identification of Cucurbita moschata hybrids.
Aiming at solving the existing issues in purity identification of Cucurbita moschata hybrids by SSR, such as complex operation and difficult application in production practice, in this study, a simple SSR-based method was established for purity identification of Cucurbita moschata hybrids. By using the established simple method, without grinding, freezing, centrifugation and drying, the genomic DNA extraction process is shorter than 3 min. Compared with the conventional method, PCR detection system and silver staining in polyacrylamide gel electrophoresis of the established method are more time-saving and The whole detection process is shorter than 4 h, and 480 samples can be detected with this method by one person in one day. The addition of the detection result exhibits a coincidence rate of 99% with field identification. The simple SSR -based method established in this study can provide basis for large-scale rapid purity identification of Cucurbita moschata hybrids.