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研究旨在通过将已筛选出对降低粪便中氮磷含量作用的细菌和真菌进行组合优化,筛选1种能有效降低保育猪粪污氮磷含量的益生菌组合,为利用益生菌降低畜禽业粪便排放导致的环境污染提供科学依据。选取16头日龄接近且体质量为57.6 kg(±3 kg)的保育猪,随机分为4个处理组,每处理组4个重复,每重复1头猪对照组饲喂基础日粮;处理组1为基础日粮+0.5%的枯草芽孢杆菌;处理组2为基础日粮+添加0.5%的枯草芽孢杆菌+0.5%的酿酒酵母菌;处理组3为基础日粮+0.5%的枯草芽孢杆菌+0.5%的酿酒酵母菌+0.5%的植物乳杆菌。结果表明:表观消化率酸不溶灰分(AIA)间接法测定,在处理组2和处理组3中,氮表观消化率提高58.65%和39.00%(P<0.01),磷表观消化率提高93.80%和71.46%(P<0.01),粗纤维消化率提高88.72%和32.51%(P<0.01),处理组2与处理组3间差异不显著(P>0.05);全收粪法测定结果中处理组3效果最明显,能有效提高氮表观消化率33.14%(P<0.01),磷表观消化率48.99%(P<0.01)。由此可见,复合益生菌能够有效提高氮、磷和粗纤维的表观消化率,减少粪便中氮和磷含量,降低畜禽粪便对环境的污染;由于AIA间接法与全收粪直接法计算方法不同,导致测定饲料的氮、磷和粗纤维表观消化率的结果存在一定区别。
The aim of this study is to optimize the combinations of bacteria and fungi that have been screened to reduce the nitrogen and phosphorus content in the faeces and to screen for a combination of probiotics that can effectively reduce nitrogen and phosphorus in pig manure so as to reduce the use of probiotics Stool emissions lead to environmental pollution to provide a scientific basis. Sixteen nursery pigs with a body mass of 57.6 kg (± 3 kg) were selected and randomly divided into four treatment groups, with four replicates per treatment group and one control group fed the basal diet. Group 1 was basal diet + 0.5% B. subtilis; treatment 2 was basal diet plus 0.5% B. subtilis plus 0.5% Saccharomyces cerevisiae; treatment 3 was basal + 0.5% Bacillus + 0.5% Saccharomyces cerevisiae + 0.5% Lactobacillus plantarum. The results showed that apparent digestibility of acid-insoluble ash (AIA), indirect digestion method, treatment group 2 and treatment group 3, the apparent nitrogen digestibility increased by 58.65% and 39.00% (P <0.01), phosphorus apparent digestibility increased 93.80% and 71.46% (P <0.01). The crude fiber digestibility increased by 88.72% and 32.51% (P <0.01), but the difference between treatment group 2 and treatment group 3 was not significant Treatment 3 showed the most obvious effect, which could effectively improve the apparent digestibility of nitrogen 33.14% (P <0.01) and the apparent digestibility of phosphorus 48.99% (P <0.01). Thus, the composite probiotics can effectively improve the apparent digestibility of nitrogen, phosphorus and crude fiber, reduce nitrogen and phosphorus content in manure and reduce environmental pollution of livestock manure; due to the direct indirect AIA indirect method and total income manure calculation Different methods led to the determination of feed nitrogen, phosphorus and crude fiber apparent digestibility results there is a certain difference.