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目的:观察大肠癌变过程中不同类型腺体组织抑癌基因p21waf1、bax、gadd45的表达及丁酸钠对其表达的影响。方法:利用二甲基肼(DMH)制作昆明小鼠大肠癌模型,通过免疫组化和RT-PCR分别检测大肠癌变过程中,小鼠大肠粘膜不同病理状态下3种抑癌基因的表达与变化。结果:在正常粘膜发展至腺瘤的过程3种基因的表达水平逐渐升高,而癌组织中3种抑癌基因的表达不同程度的降低。丁酸钠灌肠组各基因表达明显高于对照组和DMH组,其中以p21waf1的升幅最大。结论:大肠粘膜癌变与抑癌基因p21waf1、bax、gadd45的表达有关,丁酸钠可以通过调控3种抑癌基因的表达发挥抗肿瘤作用。
OBJECTIVE: To observe the expression of tumor suppressor genes p21waf1, bax and gadd45 in different types of glandular tissues and the effect of sodium butyrate on their expression in colorectal carcinogenesis. Methods: Kunming mice model of colorectal cancer was established by DMH. The expression and changes of the three tumor suppressor genes in different pathological states of colorectal mucosa were detected by immunohistochemistry and RT-PCR respectively. . Results: The expression of three genes in normal mucosa progressed to adenoma gradually increased, while the expression of the three tumor suppressor genes in cancer tissue decreased to varying degrees. Sodium butyrate enema group of gene expression was significantly higher than the control group and the DMH group, of which the largest increase in p21waf1. Conclusion: The carcinogenesis of colorectal mucosa is related to the expression of tumor suppressor genes p21waf1, bax and gadd45. Sodium butyrate can exert anti-tumor effects by regulating the expression of three tumor suppressor genes.