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以滇桂艾纳香枝叶为原料,经陶瓷膜、有机膜分离,醇沉、Sevag法除蛋白,DEAE-纤维素柱层析和SephadexG-10,SephadexG-50凝胶柱色谱纯化得到的BROS;经HPGPC检测分析表明,BROS为均一寡糖,MALDI-TOF-MS测定分子量为1314.用IR,HPLC,甲基化分析,GC-MS,NMR(1H NMR,13C NMR,1H-1HCOSY,HMQC和HMBC)等方法对BROS结构进行表征,结果表明,该寡糖由1个葡萄糖(α-D-Glcp)和7个果糖(β-D-Fruf)组成,残基间以→2)Fruf-(1→链接,分子末端链接为α-Glcp-(1→,→2)-β-D-Fruf,推出BROS的结构式为:β-D-Fruf1-(2→1)-β-D-Fruf2-(2→1)-β-D-Fruf3-(2→1)-β-D-Fruf4-(2→1)-β-D-Fruf5-(2→1)-β-D-Fruf6-(2→1)-β-D-Fruf7-(2→1)-α-D-Glcp.
The BROS obtained from the branch and leaves of D. yunnanensis was purified by ceramic membrane, organic membrane separation, alcohol precipitation, Sevag deproteinization, DEAE-cellulose column chromatography and Sephadex G-10 column chromatography. The results of HPGPC analysis showed that the BROS was a homogeneous oligosaccharide and the MALDI-TOF-MS was used to determine the molecular weight of 1314. The molecular weight was determined by IR, HPLC, methylation analysis, GC-MS, 1H NMR, 13C NMR, 1H-1HCOSY, HMQC and HMBC) were used to characterize the structure of BROS. The results showed that the oligosaccharides consisted of 1-glucose (α-D-Glcp) and 7 fructose (β-D-Fruf) 1 → 2 → 1 → β-D-Fruf. The structural formula of BROS is β-D-Fruf1- (2 → 1) -β-D-Fruf2- (2 → 1) -β-D- Fruf5- (2 → 1) -β-D-Fruf6- (2 → 1) → 1) -β-D-Fruf7- (2 → 1) -α-D-Glcp.