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结合光镜免疫细胞化学技术和图像定量分析技术，观察和比较了幼年（２～３月龄）、中年（１０～１２月龄）和老年（２２～２６月龄）雄性大鼠下丘脑促黄体生成素释放激素（ＬＨＲＨ）神经元系统的形态学特征。３个年龄组ＬＨＲＨ神经元胞体的分布模式没有区别，即大部分胞体均位于隔－视前区内。在未用秋水仙碱预处理的动物组中，中、老年大鼠ＬＨＲＨ细胞数量及其免疫反应强度比幼年组明显降低；用秋水仙碱预处理后，３个年龄组的ＬＨＲＨ细胞数量基本相等。另一方面，中、老年大鼠ＬＨＲＨ细胞表面棘样结构及其纤维膨体密度呈降低趋势。图像分析发现，３组动物ＬＨＲＨ胞体截面积基本相等，但正中隆起（ＭＥ）和终板血管器（ＯＶＬＴ）的阳性纤维密度在中、老年动物中明显降低。上述结果表明，雄性大鼠下丘脑ＬＨＲＨ神经元系统的合成和释放能力可能呈增龄性降低趋势，其原因可能主要来自传入纤维系统调节功能的下降。
Combined with light microscope immunocytochemistry and image quantitative analysis, we observed and compared the hypothalamus promotion in male (2 ~ 3 months), middle age (10 ~ 12 months) and old (22 ~ 26 months) male rats Morphological characteristics of luteinizing hormone releasing hormone (LHRH) neuronal system. There was no difference in the distribution pattern of LHRH neuronal soma in the three age groups, that is, most of the soma were located in the septal-preoptic area. In the group without colchicine pretreatment, the number of LHRH cells and the intensity of their immune responses in middle and old rats were significantly lower than those in the young group; after colchicine treatment, the numbers of LHRH cells in three age groups were almost the same . On the other hand, the spine-like structures on the surface of LHRH cells in middle-aged and old rats and their fibrous densities tended to decrease. Image analysis found that the LHRH cell body cross-sectional area of the three groups of animals was basically the same, but the positive fiber density of the middle meridian (ME) and the end-plate vasculature (OVLT) was significantly decreased in middle-aged and aged animals. The above results indicate that the synthesis and release capacity of hypothalamic LHRH neuronal system in male rats may be decreased with age, which may be mainly due to the decline of regulatory function of the afferent fiber system.